Journal: Cell Reports Medicine

Article Title: LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis

doi: 10.1016/j.xcrm.2025.102000

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Article Snippet: CTLA4; Mouse; 154Sm , Fluidigm , 3154008B; RRID:AB_3665152.

Techniques: Phospho-proteomics, Microarray, Recombinant, Lysis, Protease Inhibitor, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, TUNEL Assay, Reverse Transcription, Activity Assay, Immunoprecipitation, Extraction, Purification, In Vitro, Transfection, Sequencing, Mass Cytometry, ChIP-sequencing, Transgenic Assay, Software

Journal: Scientific Reports

Article Title: Identification of m6A methyltransferase-related WTAP and ZC3H13 predicts immune infiltrates in glioblastoma

doi: 10.1038/s41598-025-88671-4

Figure Lengend Snippet: Relative expression of genes expression in GBM tissues. Expression levels of WTAP and ZC3H13 genes were compared with those of the control group on GBM tissues, 10 GBM and adjacent tissues, respectively ( A ). The expression levels of siRNA WTAP and overexpression ZC3H13 genes ( B and C ). The expression levels of CD27, CD70, CD80, CD86, ICOS, CTLA4, and LAG3 in siRNA WTAP and overexpression ZC3H13 were demonstrated in Fig. 9D and F, respectively. The result were determined by qRT-PCR. The data are mean ± standard deviation of six replicate experiments. * P < 0.05, **P < 0.01 and ***P < 0.001 compared with the corresponding control values.

Article Snippet: After blocking with 5% nonfat milk, the membranes were immunoblotted with the primary antibodies: WTAP (A04296-2, Boster Biotech, Wuhan, China), CD27 (A01148-2, Boster Biotech), CD70 (A02853-2, Boster Biotech), CD80 (A00196-3, Boster Biotech), CD86(BM4121, Boster Biotech), ICOS (A00291-2, Boster Biotech), CTLA4 (A00020-1, Boster Biotech), LAG3 (M02869-2, Boster Biotech), Beta-actin (BM3873, Boster Biotech).

Techniques: Expressing, Control, Over Expression, Quantitative RT-PCR, Standard Deviation

Journal: STAR Protocols

Article Title: Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry

doi: 10.1016/j.xpro.2024.103038

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Article Snippet: Human CTLA4/CD152(14D3)-161Dy (1:100) , Standard BioTools , Cat#3161004B.

Techniques: Recombinant, Labeling, Blocking Assay, Software, Cytometry, Flow Cytometry, Single-cell Analysis, Gene Expression

Journal: iScience

Article Title: Single cell characterization of blood and expanded regulatory T cells in autoimmune polyendocrine syndrome type 1

doi: 10.1016/j.isci.2024.109610

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Article Snippet: CD152 (CTLA4) , Fluidigm , 3161004B; RRID: AB_2687649.

Techniques: Isolation, Amplification, Hybridization, Cell Isolation, Staining, RNA Sequencing, Gene Expression, Mass Cytometry, Flow Cytometry, Software